These approaches like northern, western, and southern, are appropriate for diverse types of macromolecules such as DNA, RNA, lipids, and proteins.
by means of probe we have to distinguish the concern molecule.
Recognizing and determining specific proteins in complex biological mixtures, like blood, have extensive significant objectives in scientific and diagnostic practice.
In recent times the Identification of abnormal genes in genomic DNA has developed progressively significant in clinical investigation and genetic analysis.
Blotting techniques are castoff to recognize distinctive proteins or nucleic acid arrangements.
WESTERN BLOTTING
W. Neal Burnette in 1981 developed Western blotting technique.
This technique is depleted for recognition and examination of protein in a particular sample.
It includes the transmission of electrophoresed protein bands from a polyacrylamide gel on to a membrane of nitrocellulose or nylon, to which they bind strongly.
